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Mineral deposition was monitored using alizarinred as a vital stain for calcium.
2
Mineralized matrix formation was visualized using alizarinred S staining.
3
Approach and results: Arterial calcium accumulation was evaluated using alizarinred stain and atomic absorption spectrometry.
4
The results of alizarinred staining and ALP detection showed that SpA treatment suppressed the osteogenic differentiation of hBMSCs.
5
Cell counting kit-8 activity was examined to test cell proliferation, and alkaline phosphatase assays and alizarinred S staining were conducted to examine differentiation.